The present day Biochemical Oxygen Demand Testing (BOD Test) of water is quite extensively used in performances related to ecological expertise and control of the environment. This kind of research allows specifying biochemical oxygen demand in natural and waste waters and repels the mutual rate of water pollution.
The rate of water property which describes the aggregated substance of organic matters in the water is termed biochemical oxygen demand (BOD).
Specification of BOD reposes on measurement of concentration of dissolved oxygen in a water test without delay after withdrawal and as well after incubation of a probe. Cultivation of a sample is executed without air ingress ina special oxygen bottle during the time which is needed for passing the reaction of biochemical oxidation. Since the pace of biochemical reaction depends on the temperature, cultivation is executed in permanent temperature mode (about 20±1)°C, in which connection exactness of BOD analysis hinges on exactness of sustentation the temperature. BOD is generally defined in the middle of the 5-day time of cultivation, but the substance of some combinations is much more informatively described with the mensuration of 10-day BOD or full-term acidification BOD. The error in designating of BOD can be made either by lighting of a trial. This lighting exerts on vital functions of microorganisms and is able to cause photochemical oxidation in some cases. That’s why the cultivation is implemented without ingress of light (in a black room). In the majority of cases as well special chemical reagents and BOD Incubators are required for the execution of BOD test.